Journal: Journal of nanobiotechnology

Article Title: ROS-responsive liposomes as an inhaled drug delivery nanoplatform for idiopathic pulmonary fibrosis treatment via Nrf2 signaling.

doi: 10.1186/s12951-022-01435-4

Figure Lengend Snippet: Fig. 7 DTP@DMF NPs suppress fibrosis and macrophage accumulation in lung tissue via Nrf2 signaling. a Representative H&E and Masson’s trichrome staining and b the severity score of fibrosis after DMF, DP@DMF NPs and DTP@DMF NPs treatment (n = 5). c α-SMA and d collagen Ia1 protein levels in fibrotic tissue (n = 3). e Nrf2 and f HO-1 levels in fibrotic tissue (n = 3). g The Nrf2 (red) and HO-1 (red) expression in F4/80+ macrophages (green) detected by immunofluorescence analysis. h Immunohistochemistry of F4/80+, CD86+, and CD206+ macrophages in pulmonary tissues, and i–m quantification of total macrophages and M1 and M2 phenotypes (n = 5). n TGF-β levels in BALF, o SOD and p MDA levels in tissue (n = 5). Statistical analyses were performed via one-way ANOVA with S–N-K post hoc analysis. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Antibodies against Nrf2, collagen Ia1, α-smooth muscle actin (SMA), F4/80, and CD206 were purchased from Cell Signaling Technology (MA, USA).

Techniques: Staining, Expressing, Immunofluorescence, Immunohistochemistry

Journal: Journal of nanobiotechnology

Article Title: Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flaps.

doi: 10.1186/s12951-024-02533-1

Figure Lengend Snippet: Fig. 6 3D-ABs suppress oxidative stress and cell death, and stimulated angiogenesis in ischaemic flaps. (A) F-CHP staining for damaged collagen detec tion in the skin on POD7. Scale bars, 100 μm. (B) The three groups’ quantified F-CHP intensity (n = 6). (C) CD31 and α-SMA IF staining in the flap on POD7. Scale bars, 50 μm. (D) Quantified CD31/α-SMA-positive blood vessel density among the three groups (n = 6). (E) Dead cells in flap tissue sections on POD7 under the detection of TUNEL staining. Scale bar, 20 μm. (F) TUNEL-positive cell percentage in the dermal layer, quantified for each of the three groups (n = 6). (G) DHE staining of the skin tissues on POD7 among the three groups. Scale bars, 50 μm. (H) Quantified DHE among the three groups (n = 6). SEM error bars are used. Significance (*): p value < 0.05; equal variances ANOVA with LSD post hoc analysis or unequal variances Dunnett’s T3 technique

Article Snippet: The primary antibodies utilized were specific to CD31 (1:200), CD68 (1:200), α-SMA (1:200; Proteintech, 67735-1-Ig), iNOS (1:200), and Arg1 (1:200).

Techniques: Staining, TUNEL Assay